In this video, we demonstrate the step-by-step process of isolating viable rat cardiomyocytes — a fundamental technique in cardiac electrophysiology and calcium imaging studies. The isolation of single, healthy cardiomyocytes enables precise investigation of cellular electrophysiological properties and intracellular calcium dynamics.
This procedure is essential for researchers studying cardiac function, ion channel behavior, and excitation–contraction coupling. It also provides a reliable platform for testing pharmacological interventions and understanding the mechanisms underlying cardiac diseases at the cellular level.
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